Imran Rashid Rajput
Shantou University, China
Title: Cell culture and Molecular approach to decrease massive stranding of Cetaceans
Biography
Biography: Imran Rashid Rajput
Abstract
Statement of the Problem: Rapid declining population of cetacean is moving towards their extinctions, and protection department and researchers could not find the biological cause despite of physical injury and plastic materials in cetacean stomach. The sampling of cetaceans is restricted and field trails are likely to be impossible in the sea or oceans to diagnose cetaceans. Our presentation describes the state of art to know the unknow reason of cetacean death. Skin biopsy is only possible to some extent for sample collection, and we suggest that fibroblast cells culturing is possible to grow in the laboratory. These fibroblast cells can be genetically modified and converted into immortalized cell line. In the meantime, primary cells can also be converted into neuron and hepatocyte like cells by inducing some specific factors to conduct neurological and hepatological effects and response studies. These can be used to diagnose and estimate the molecular changes in the cetacean body. Several studies in the human has proven that fibroblast cells express about 100 genes related to neurodegenerative change and hepatic cells response related genes also found in fibroblast cells. This presentation demonstrates the new strategy to cetacean in the laboratory.