Marc Wold
University of Iowa, USA
Biography
Marc Wold My laboratory has been study cellular DNA metabolism for more than 20 years. My lab has experience with a wide-range of biochemical, molecular and cellular approaches for analyzing DNA metabolism in vitro and in vivo. We have extensive experience with purification and biochemical analysis of proteins involved in DNA replication and repair. We have either developed or applied many methods for examining DNA binding and functional analysis of RPA in DNA replication and repair. We also carried out structural analysis of RPA and in cell methods for analyzing the function of different forms of RPA or other proteins in human cells. These studies have developed methodology for determining the functions of RPA in cellular DNA replication, DNA repair (including methods for analyzing the efficiency of specific DNA repair pathways) and checkpoint activation. My lab has generated and purified a large number of mutant forms of RPA and analyzed the mechanism of RPA-DNA interactions. We have domain deletion and point mutations at disrupted the function of individual domains of RPA, alter DNA
Abstract
Abstract : DNA repair depends on specialized DNA-binding by replication protein A